000 02554cam a2200433 i 4500
008 151119s2013 ne a b 001 0 eng
010 _a2013-002493
020 _a9780124158061
035 _avtls011327312
039 9 _y201511191121
_zmschk03
040 _aDNLM/DLC
_beng
_cDLC
_dSTUSCHK1
_erda
042 _apcc
060 1 0 _aQU 25
080 0 0 _a543/.84
_223
245 0 0 _aLiquid chromatography
_bApplications
_cSalvatore Fanali, Paul R. Haddad, David Lloyd, Colin F. Poole, Peter Schoenmakers, editors
246 1 _aApplications
264 1 _aAmsterdam
_bElsevier
_c2013
300 _axiii, 667 p.
_bill.
_c24 cm.
336 _atext
_2rdacontent
337 _aunmediated
_2rdamedia
338 _avolume
_2rdacarrier
504 _aIncludes bibliographical references and index.
520 _a"Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyze specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including bioaffinity chromatography, immunoaffinity chromatography, dye-ligand and biomimetic affinity chromatography, immobilized metal-ion affinity chromatography, and analytical affinity chromatography. The types of ligands utilized in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers"--Provided by publisher.
650 1 2 _aChromatography, Affinity
_xmethods.
650 2 2 _aProteins
_xchemistry.
700 1 _aHaddad, Paul R.
_4edt
700 1 _aPoole, Colin F.
_4edt
700 1 _aSchoenmakers, Peter
_4edt
700 1 _aLloyd, David
_4edt
700 1 _aFanali, Salvatore
_4edt
906 _a7
_bcbc
_corignew
_d1
_eecip
_f20
_gy-gencatlg
910 _aSCHK95
919 _a978-0--12-415806-1
925 0 _aacquire
_b1 shelf copy
_xpolicy default
925 1 _aacquire
_b2 shelf copies
_xpolicy default
955 _drd13 2013-01-24
_wrd13 2013-01-29
_erf17 2013-10-23
_frf17 2013-10-23 to CALM
_trf17 2013-10-23 to CALM
985 _aNLMCIP
_d2013-01-24
999 _c7074
_d7074