Brown, T. A.

Gene cloning and DNA analysis : an introduction / T.A. Brown. - 5th ed. - Oxford, Malden, MA Blackwell Pub. 2006. - xx, 386 p. ill. 25 cm.

Includes bibliographical references and index.

PART 1: THE BASIC PRINCIPLES OF GENE CLONING AND DNA ANALYSIS: Why gene cloning and DNA are important -- Vectors for gene cloning: plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for E. coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- PART 2: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN RESEARCH: Studying gene location and structure -- Studying gene expression and function -- Studying genomes -- PART 3: THE APPLICATION OF GENE CLONING AND DNA ANALYSIS IN BIOTECHNOLOGY: Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology -- Glossary.

1405111216 (pbk. : alk. paper) 9781405111218 (pbk. : alk. paper)

2005-006606


Molecular cloning.
Nucleotide sequence.
DNA--Analysis.
Cloning, Molecular.
DNA, Recombinant--analysis.
Sequence Analysis, DNA.

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